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HOW TO DETERMINE PCV (PACKED CELL VOLUME)

The Packed Cell Volume (PCV) Test 1. Objective: This is one of the oldest tests done in medical practice. The objective of this test was to determine the percentage of red blood cells (RBCs) in whole blood. It  also helped to assess the blood’s capacity to carry oxygen. ________________________________________ 2. Principle: The test was based on the principle that when blood was centrifuged, the red blood cells settled at the bottom of the tube, forming a packed column. The ratio of the packed RBC column to the total blood column gave the packed cell volume (PCV), expressed as a percentage. ________________________________________ 3. Materials: • Microhematocrit capillary tubes • Microhematocrit centrifuge • Plasticine or sealing clay • Microhematocrit reader • Lancet • Alcohol swab • Cotton ________________________________________ 4. Procedure (Microhematocrit Method): 1. The fingertip was cleaned with an alcohol swab and pricked with a sterile lancet. 2. Blood was collected into ...
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